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A Simple High-Performance Liquid Chromatography for Determining Lapatinib and Erlotinib in Human Plasma
Masahiro Ohgami1, Masato Homma, Yoshiharu Suzuki
1*Department of Pharmaceutical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan; †Department of Pharmacy, Ibaraki Prefectural Central Hospital, Kasama, Japan; ‡Department of Pharmacy, University of Tsukuba Hospital, Tsukuba, Japan; §Department of Medical Oncology, Ibaraki Prefectural Central Hospital, Kasama, Japan; ¶Department of Respiratory Medicine, Ibaraki Prefectural Central Hospital, Kasama, Japan; and ‖Department of Breast and Endocrine Surgery, University of Tsukuba, Tsukuba, Japan.
Background:
Lapatinib and erlotinib are used for cancer treatment, showing large interindividual variability. Therapeutic drug monitoring may be useful for assessing the clinical outcomes and adverse events. A simple high-performance liquid chromatography UV method was developed for the determination of lapatinib and erlotinib in human plasma.
Methods:
An aliquot of plasma sample spiked with internal standard was treated with acetonitrile to precipitate the proteins. Lapatinib and erlotinib were separated on an octadecylsilyl silica gel column using a mobile phase consisting of acetonitrile, methanol, water, and trifluoroacetic acid (26:26:48:0.1) pumped at a flow rate of 1.0 mL/min. The detection wavelength was set at 316 nm.
Results:
The calibration curves for lapatinib and erlotinib were linear (r = 0.9999) in the range of 0.125-8.00 mcg/mL. The extraction recoveries for both lapatinib and erlotinib at the plasma concentration of 0.125-8.00 mcg/mL were higher than 89.9% with coefficients of variation less than 3.5%. The coefficients of variation for intraday and interday assays of lapatinib and erlotinib were less than 5.1% and 6.1%, respectively.
Conclusions:
The present method can be used for blood concentration monitoring for lapatinib or erlotinib in exactly the same conditions.
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