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Temperature mapping using molecular diffusion based fluorescence thermometry via simultaneous imaging of two

Chen Chen, Zhidong Du, Jicheng Wang

|November 19, 2016

Summary

A novel optical technique uses fluorescent molecule motion to map 2D temperature distributions in liquids. This two numerical apertures (2NA) method offers robust and versatile fluorescence thermometry.

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Area of Science:

Optics and Photonics
Physical Chemistry
Materials Science

Background:

Accurate temperature mapping in liquid solutions is crucial for various scientific disciplines.
Existing thermometry methods face limitations in resolution, robustness, and applicability to diverse materials.

Purpose of the Study:

To introduce a new optical technique for high-resolution, two-dimensional temperature mapping in liquid solutions.
To demonstrate the robustness and versatility of the proposed fluorescence thermometry method.

Main Methods:

Simultaneously capturing fluorescence images at different numerical apertures (NAs).
Utilizing the temperature-dependent orientation of emission dipoles to infer temperature.
Employing a two numerical apertures (2NA) configuration to validate the concept.

Main Results:

The 2NA technique effectively maps 2D temperature distributions.
The method demonstrates robustness against photobleaching, illumination variations, and molecule distribution.
The technique is applicable to polarizing materials and inherently filters background emission.

Conclusions:

The developed 2NA fluorescence thermometry technique provides a novel and powerful tool for temperature measurement.
This method offers significant advantages in terms of robustness, versatility, and background suppression.
The technique is poised to advance fluorescence thermometry applications across various fields.