Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

X-ray Imaging01:24

X-ray Imaging

10.9K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
10.9K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

5.1K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
5.1K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

14.9K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
14.9K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.8K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exposure control at the first dark-field chest radiography demonstrator system.

Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)·2025
Same author

Finite element fracture load analysis and dark-field X-ray imaging of osteoporotic and healthy vertebrae in human lumbar spine specimens.

BMC musculoskeletal disorders·2025
Same author

Parity-dependent state transfer for direct entanglement generation.

Nature communications·2025
Same author

[New awareness for zoonoses using the example of rat bite fever : Case report and literature review].

Innere Medizin (Heidelberg, Germany)·2024
Same author

Analyzer-free hard x-ray interferometry.

Physics in medicine and biology·2024
Same author

Assessment of the Factors Influencing the Recording Performance of Circumneural Electrodes.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2023
Same journal

Long-term stabilization of intensity-difference squeezing from four-wave mixing in rubidium vapor.

Optics express·2026
Same journal

Robust 3D topography measurement of large-range high-aspect-ratio structures based on dual-domain statistical filtering in SD-OCT.

Optics express·2026
Same journal

Broadband transmissive terahertz metasurface for simultaneous quad-mode OAM multiplexing.

Optics express·2026
Same journal

Leveraging two-dimensional materials for high-sensitivity optical sensors: quasi-bound states in the continuum within hybrid metasurfaces.

Optics express·2026
Same journal

Resolution investigation for dual-spherical-wave optical scanning holographic microscopy: methods and performance.

Optics express·2026
Same journal

Robustness of parallel subnetwork-filtered diffractive deep neural networks.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Mar 12, 2026

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
10:12

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

Published on: June 19, 2018

9.7K

Two-shot X-ray dark-field imaging.

M Marschner, L Birnbacher, K Mechlem

    Optics Express
    |November 19, 2016
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a new method for faster, lower-dose dark-field imaging using grating-based phase-contrast imaging. The technique accurately retrieves dark-field signals, even in low photon conditions, advancing clinical applications.

    More Related Videos

    High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
    07:48

    High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

    Published on: September 30, 2022

    1.7K
    Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography
    10:18

    Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

    Published on: February 21, 2017

    8.9K

    Related Experiment Videos

    Last Updated: Mar 12, 2026

    Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
    10:12

    Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

    Published on: June 19, 2018

    9.7K
    High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
    07:48

    High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

    Published on: September 30, 2022

    1.7K
    Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography
    10:18

    Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

    Published on: February 21, 2017

    8.9K

    Area of Science:

    • Medical Imaging
    • Physics
    • Biomedical Engineering

    Background:

    • Grating-based phase-contrast imaging is crucial for medical diagnostics.
    • Current dark-field retrieval methods can be time-consuming and require high radiation doses.
    • Limitations exist in conventional techniques, especially with low photon statistics.

    Purpose of the Study:

    • To develop a novel, efficient acquisition scheme for dark-field data retrieval.
    • To improve the accuracy of dark-field signal recovery, particularly in challenging conditions.
    • To extend the proposed method to tomographic imaging for enhanced clinical feasibility.

    Main Methods:

    • A novel two-phase-step acquisition scheme was developed for dark-field data retrieval.
    • The method was validated for its ability to handle low photon statistics.
    • The scheme was successfully extended to tomographic investigations using iterative reconstruction.

    Main Results:

    • The proposed method requires only two phase steps, reducing acquisition time and dose.
    • Accurate dark-field signal retrieval was achieved, outperforming conventional methods in low photon statistics scenarios.
    • Successful implementation of two-shot dark-field imaging in tomographic reconstruction was demonstrated.

    Conclusions:

    • The novel two-shot acquisition scheme offers a significant advancement in dark-field imaging.
    • This technique enhances the accuracy and efficiency of dark-field data retrieval.
    • The findings represent a crucial step towards the clinical application of dark-field tomography.