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Related Concept Videos

Proteomics01:33

Proteomics

10.0K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Transcription Factors02:16

Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Transcription Factors02:16

Transcription Factors

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26.4K
General Transcription Factors01:30

General Transcription Factors

7.4K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
7.4K
Ribosome Profiling02:24

Ribosome Profiling

4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.3K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

12.7K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
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Related Experiment Video

Updated: Mar 11, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
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Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

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Transcription factor proteomics-Tools, applications, and challenges.

Jovan Simicevic1,2, Bart Deplancke1

  • 1Laboratory of Systems Biology and Genetics, Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), and Swiss Institute of Bioinformatics, Lausanne, Switzerland.

Proteomics
|November 19, 2016
PubMed
Summary
This summary is machine-generated.

Quantitative proteomics now allows precise measurement of transcription factors (TFs), moving beyond gene expression data. This shift reveals TF copy number dynamics and their regulatory roles in biological processes and diseases.

Keywords:
EnrichmentMass spectrometryQuantificationTechnologyTranscription factor

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Area of Science:

  • Molecular Biology
  • Proteomics
  • Genetics

Background:

  • Transcription factors (TFs) are crucial DNA-binding proteins regulating cellular states.
  • Understanding TF function is limited by difficulties in quantifying their low-abundance proteins.
  • Current reliance on gene expression data provides an indirect measure of TF protein levels.

Purpose of the Study:

  • To review recent advancements in mass spectrometry-based proteomics for TF quantification.
  • To highlight novel strategies for sensitive detection and measurement of TFs.
  • To explore the potential of quantitative TF proteomics in understanding cellular regulation.

Main Methods:

  • Mass spectrometry (MS) technologies for protein detection.
  • Sensitive methodologies specifically designed for TF analysis.
  • Quantitative proteomics approaches for TF copy number determination.

Main Results:

  • Emerging MS technologies enable a transition from qualitative to quantitative TF analysis.
  • New methodologies facilitate the detection of low-abundance TFs.
  • Quantitative data offers insights into TF copy number fluctuations and their functional relevance.

Conclusions:

  • Quantitative proteomics is revolutionizing the study of transcription factors.
  • This paradigm shift promises to uncover dynamic TF functions and regulatory mechanisms.
  • Advances in TF proteomics will yield new insights into fundamental and disease-related biological processes.