Related Experiment Video

Updated: Mar 11, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

9.8K

Association of High-Density Lipoprotein Subclasses with Chronic Kidney Disease Progression, Atherosclerosis, and

Eiichiro Kanda1,2, Masumi Ai3, Mitsuyo Okazaki4

  • 1Department of Nephrology, Tokyo Kyosai Hospital, Meguroku, Tokyo, Japan.

Plos One
|November 19, 2016
PubMed

Insights

High-density lipoprotein (HDL) subclasses are linked to chronic kidney disease (CKD) progression and atherosclerosis. Specific HDL cholesterol proportions correlate with kidney function decline and arterial stiffness, varying by CKD stage.

Area of Science:

  • Nephrology
  • Cardiology
  • Lipidology

Background:

  • Atherosclerosis is a common complication in chronic kidney disease (CKD), driven by dyslipidemia and CKD-mineral and bone disorder.
  • High-density lipoproteins (HDLs) have diverse subclasses with altered composition and function in CKD.
  • Investigating HDL subclasses is crucial for understanding CKD progression and associated atherosclerosis.

Purpose of the Study:

  • To explore the role of lipoprotein subclasses in CKD progression.
  • To examine the association between lipoprotein subclasses and atherosclerosis.
  • To investigate the relationship between lipoprotein subclasses, Klotho, and fibroblast growth factor 23 (FGF23).

Main Methods:

  • Prospective cohort study of 71 CKD patients in Japan.
  • Quantification of cholesterol proportion and lipoprotein particle numbers across 20 lipoprotein fractions using high-performance gel permeation chromatography.
  • Multivariate regression analysis to identify associations adjusted for baseline characteristics.

Main Results:

  • Higher particle numbers in small HDLs were observed in Stage 4 CKD compared to Stage 5 CKD.
  • In Stage 4 CKD, cholesterol proportions in very small HDLs correlated with estimated glomerular filtration rate (eGFR) change rate and ankle-brachial index (ABI).
  • In Stage 5 CKD, serum soluble α-Klotho levels were associated with lipoprotein particle numbers in very small HDLs.

Conclusions:

  • HDL subclasses are significantly associated with CKD progression.
  • Specific HDL subclasses correlate with atherosclerosis markers like ABI.
  • The relationship between HDL subclasses, CKD progression, and Klotho levels is stage-specific in CKD patients.
Abstract

More Related Videos

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles09:15

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles

Published on: November 10, 2017

15.2K
Quantification of Atherosclerosis in Mice06:59

Quantification of Atherosclerosis in Mice

Published on: June 12, 2019

40.9K

Related Concept Videos

Chronic Kidney Disease I: Introduction01:25

Chronic Kidney Disease I: Introduction

Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage renal disease. At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate for...
936
Chronic Kidney Disease II: Clinical Manifestations01:24

Chronic Kidney Disease II: Clinical Manifestations

Chronic Kidney Disease (CKD) progressively impairs multiple body systems due to the accumulation of uremic toxins, which disrupt cellular functions across various organs.Neurologic symptomsNeurologic symptoms often arise early in CKD, as uremic toxin buildup drives changes in cognitive and motor functions. Patients frequently experience fatigue, headache, confusion, difficulty concentrating, and, in severe cases, seizures. Peripheral neuropathy commonly manifests as burning sensations in the...
864
Atherosclerosis I: Introduction01:30

Atherosclerosis I: Introduction

Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...
1.7K
Chronic Kidney Disease III: Interprofessional Care01:28

Chronic Kidney Disease III: Interprofessional Care

Chronic kidney disease (CKD) requires collaborative and comprehensive management. CKD progresses through stages and can lead to end-stage kidney disease (ESKD) if untreated. Interprofessional collaboration and patient education are crucial, enabling patients to manage their health and improve their quality of life.Diagnostic approach for chronic kidney diseaseThe diagnosis of CKD primarily focuses on the glomerular filtration rate (GFR), which assesses kidney function by measuring how well...
565
Atherosclerosis III: Management01:26

Atherosclerosis III: Management

Management of atherosclerosis involves an integrated strategy encompassing pharmacological treatment, surgical interventions, lifestyle changes, and nutrition therapy to address the multifactorial nature of the disease.Pharmacological TherapyA cornerstone of atherosclerosis management is the use of pharmacological agents. Statins, such as atorvastatin, are pivotal in inhibiting HMG-CoA reductase, an enzyme that catalyzes an initial step in cholesterol synthesis in the liver. This reduction in...
541
Coronary Artery Disease II: Pathophysiology01:26

Coronary Artery Disease II: Pathophysiology

Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...
862