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Phosphodiester Linkages

Phosphodiester Linkages

Overview
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...

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Updated: Mar 11, 2026

Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction

Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction

Published on: September 14, 2018

Tetrazine-Responsive Self-immolative Linkers.

Kevin Neumann¹, Sarthak Jain¹, Alessia Gambardella¹

¹EaStCHEM School of Chemistry, University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh, EH9 3FJ, UK.

Chembiochem : a European Journal of Chemical Biology

|November 19, 2016

Summary

This summary is machine-generated.

Researchers developed a novel method for activating molecules using a self-immolative linker and tetrazine. This technique enables efficient decaging and controlled drug delivery, like doxorubicin, within cells.

Keywords:

Diels-Alder reaction doxorubicin nanoparticles prodrugs tetrazine

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Last Updated: Mar 11, 2026

Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction

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Area of Science:

Chemical Biology
Organic Chemistry
Drug Delivery Systems

Background:

Molecules activated by external stimuli have broad applications.
Controlled release of active compounds is crucial for targeted therapies.

Purpose of the Study:

To report an efficient method for molecular decaging using a self-immolative linker.
To demonstrate the controlled delivery of doxorubicin in a cellular context.

Main Methods:

Activation of a self-immolative linker via tetrazine.
Application of water-soluble and stable tetrazine as a chemical stimulus.
Monitoring the decaging of various moieties.

Main Results:

Highly efficient decaging of diverse molecular moieties was achieved.
Tetrazine-mediated activation proved effective and stable.
Successful controlled delivery of doxorubicin within cells was demonstrated.

Conclusions:

The developed self-immolative linker system offers a robust platform for molecular activation.
This approach facilitates precise control over the release of chemical entities.
The method holds promise for advanced drug delivery and chemical biology applications.