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Protein Kinase C-delta Inhibitor Peptide Formulation using Gold Nanoparticles
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Array-based functional peptide screening and characterization of gold nanoparticle synthesis.

Masayoshi Tanaka1, Shun Hikiba2, Kiyoto Yamashita2

  • 1Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, 2-12-1, O-okayama, Meguro-ku, Tokyo 152-8552, Japan; JST ImPACT, Japan.

Acta Biomaterialia
|November 21, 2016
PubMed
Summary

Researchers identified specific tryptophan-based peptide motifs that control gold nanoparticle synthesis. These peptides enable precise, ambient condition regulation of gold nanoparticle production and integration for nanotechnological devices.

Keywords:
Au(III) reducing peptideBi-functional peptideGold nanoparticlePeptide array

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Peptide Chemistry

Background:

  • Biomineralization inspires using biological molecules for controlled nanomaterial synthesis.
  • Short peptides show potential for regulating inorganic material production, but variety is limited.
  • Developing ambient condition techniques for functional nanosized material production is crucial.

Purpose of the Study:

  • To identify and characterize peptides with gold nanoparticle (AuNP) binding activity.
  • To explore the role of specific peptide motifs in AuNP synthesis and reduction.
  • To enable peptide-mediated precise regulation of AuNP synthesis and integration.

Main Methods:

  • Peptide array synthesis and screening of approximately 1800 peptides for AuNP binding.
  • Analysis of peptide sequences, focusing on tryptophan-based motifs (W[Xn]W, H[Xn]W, W[Xn]H).
  • Characterization of truncated peptide derivatives to confirm functional motifs.

Main Results:

  • Identified ~1800 peptides with varying AuNP binding activities.
  • Discovered that 46 of 50 highest-affinity peptides contain tryptophan-based motifs.
  • Observed bi-functionality (AuNP binding and Au(III) reduction) in three top-binding peptides.

Conclusions:

  • Tryptophan-based motifs are critical for peptide-AuNP binding and function.
  • Peptide-mediated synthesis offers precise control over AuNP properties in ambient conditions.
  • Findings facilitate site-dependent AuNP integration in nanotechnological devices.