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Artefactual low lymphocyte activity caused by platelet contamination in the mononuclear cell preparations
1Unitat de Recerca Biomedica, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
Abstract:
Platelet (PLT) contamination in preparations of mononuclear cells (MNC) produces a significant decrease (P less than 0.02) in lymphoproliferative activity and interleukin 2 (IL2) production. This effect was observed in MNC preparations from either healthy blood donors or polyglobulic patients. In a dose-response curve carried out with increasing PLT number per MNC, progressive inhibition was found, reaching to 62.6% and 49.5%, respectively, to phytohemagglutinin and IL2 lymphoproliferative response and 87.8% in IL2 production, at 80 PLTs/MNC.
Insights
Platelet (PLT) contamination significantly reduces mononuclear cell (MNC) lymphoproliferative activity and IL2 production. Even low levels of PLT contamination inhibit immune responses in blood samples from healthy donors and patients.
Area of Science:
- Immunology
- Cell Biology
Background:
- Mononuclear cells (MNCs) are crucial for immune responses.
- Platelets (PLTs) are known to influence immune cell function.
Purpose of the Study:
- To investigate the impact of platelet contamination on mononuclear cell lymphoproliferative activity and IL2 production.
Main Methods:
- MNC preparations from healthy donors and polyglobulic patients were analyzed.
- Dose-response curves were used to assess the effect of increasing PLT numbers per MNC.
Main Results:
- PLT contamination significantly decreased lymphoproliferative activity and IL2 production (P < 0.02).
- Progressive inhibition was observed with increasing PLT numbers.
- At 80 PLTs/MNC, phytohemagglutinin response decreased by 62.6%, IL2 response by 49.5%, and IL2 production by 87.8%.
Conclusions:
- Platelet contamination negatively impacts MNC function, specifically lymphoproliferation and IL2 production.
- These findings are relevant for both healthy individuals and patients with conditions like polyglobulia.
- Careful control of PLT levels in MNC preparations is essential for accurate immunological assessments.