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Published on: December 15, 2017
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Current applications and different approaches for microbial l-asparaginase production
Jorge Javier Muso Cachumba1, Felipe Antonio Fernandes Antunes1, Guilherme Fernando Dias Peres1
1Universidade de São Paulo, Escola de Engenharia de Lorena, São Paulo, SP, Brazil.
Summary
L-asparaginase is an enzyme crucial for treating leukemia and reducing acrylamide in food. Research focuses on improving its biotechnological production and expanding its applications.
Area of Science:
- Biochemistry
- Enzymology
- Biotechnology
Background:
- L-asparaginase (EC 3.5.1.1) catalyzes asparagine hydrolysis, yielding aspartic acid and ammonium.
- This enzyme is found in various organisms but not humans, and is vital in chemotherapy for lymphoproliferative disorders like acute lymphoblastic leukemia (ALL).
- It also serves a key role in the food industry by reducing acrylamide formation in fried products.
Purpose of the Study:
- To review the applications of L-asparaginase.
- To discuss its production methods using microorganisms.
- To highlight current research, limitations, and challenges for industrial production.
Main Methods:
- Literature review of L-asparaginase applications and production.
- Analysis of biotechnological production methods, primarily using bacteria.
- Identification of limitations and areas for future research.
Main Results:
- L-asparaginase has established roles in cancer therapy and food processing.
- Biotechnological production, mainly by bacteria, is the current standard.
- Significant potential exists for improving yields and exploring novel microbial sources.
Conclusions:
- Further research is needed to optimize L-asparaginase production for industrial scalability.
- Exploring diverse microorganisms can enhance enzyme efficiency and broaden applications.
- Addressing production challenges is key to cost-effective industrial implementation.
