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Updated: Mar 11, 2026
Isolation and Characterization of a Head and Neck Squamous Cell Carcinoma Subpopulation Having Stem Cell Characteristics
Published on: May 11, 2016
Expression of stem cell markers in oral cavity and oropharynx squamous cell carcinoma
Flávia Paiva Prudente de Moraes1, Silvia Vanessa Lourenço2, Renata Carolina Fraga Ianez1
1International Research Center, AC Camargo Cancer Center, São Paulo, SP, Brazil; Department of Pathology, AC Camargo Cancer Center, São Paulo, SP, Brazil.
Objectives:
The aim of this study was to analyze the expression of CD24, CD44, CD133, ALDH1, CD29 (integrin-β1), and Ki-67 in squamous cell carcinoma of the oral cavity and oropharynx.
Study Design:
Fifty-two tumors and 21 metastatic lymph nodes were evaluated by using immunohistochemistry.
Results:
Seven of 52 cases (13.5%) showed positive cytoplasmic staining of aldehyde dehydrogenase 1; integrin-β1 was expressed in 45 of 50 cases (90%); 30 of 52 cases (57.7%) had positive membranous staining of CD44; CD24 was expressed in 44 of 50 cases (88%); and three of 52 cases (5.8%) stained positively for membranous CD133. Median proliferation rate, measured by Ki-67, was 37.1% for tumors. Five-year cancer-specific survival rates for the CD44-negative and CD44-positive groups were 74% and 38%, respectively, although this difference did not reach statistical significance (P = .052).
Conclusions:
Our study demonstrated the expression of putative stem cell markers in squamous cell carcinoma of the oral cavity and oropharynx, with participation of CD44-positive cells in association with poor survival outcome.
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