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Published on: April 22, 2019
Bone-remodeling-related gene expression in oral squamous cell carcinoma: impact on mandibular invasion and overall
Érica Fernanda Patricio1, Gustavo Davi Rabelo2, Carlos Henrique Horst Bianchin2
1A.C.Camargo Cancer Center (Stomatology Department), São Paulo (São Paulo), Brazil.
Objectives:
Bone invasion by oral squamous cell carcinoma (OSCC) activates the remodeling process with the participation of RANK/RANKL/OPG and Interleukin 6 (IL-6) genes. This study aimed to evaluate gene expressions of RANK, RANKL, OPG, and IL-6 in OSCC associated with mandibular invasion (MI) and to identify their influence on patients' overall survival (OS).
Materials And Methods:
A cross-sectional study selected patients treated with mandibulectomy for OSCC in a reference center, resulting in two hundred and twenty individuals. Forty age-match patients with material for molecular analysis were included in the study to evaluate RANK, RANKL, OPG, and IL6 expression analysis, divided into 2 groups: MI (n = 20) and no invasion (NI, n = 20). Tumor mRNA expression of the selected genes was determined by real-time polymerase chain reaction (calibrated by endogenous GAPDH and a control pool of non-neoplastic RNA).
Results:
Gene expression analysis revealed 16 of 36 cases (44%) presenting downregulation for RANK; 28 of 37 samples (75%) with overexpression for RANKL; 19 of 35 samples (54%) with downregulation for IL-6; and 16 of 37 samples (43%) with normal expression for OPG. IL-6 expression was associated with MI, and all the genes were correlated with each other (p < 0.05). Multivariate Cox regression revealed an association of MI and perineural invasion with OS, and higher OS rate was associated with IL-6 downregulation (p = 0.053, 108.5 months).
Conclusions:
Mandibular invasion may impact overall survival and is associated with IL-6 tumor expression. Downregulation of IL-6 by malignant cells is associated with higher overall survival.
