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Updated: Mar 11, 2026
Folding and Characterization of a Bio-responsive Robot from DNA Origami
Published on: December 3, 2015
Cellular delivery of enzyme-loaded DNA origami
Ari Ora1, Erika Järvihaavisto1, Hongbo Zhang2
1Biohybrid Materials, Department of Biotechnology and Chemical Technology, Aalto University, P. O. Box 16100, FI-00076 Aalto, Finland. mauri.kostiainen@aalto.fi veikko.linko@aalto.fi.
Abstract:
In this communication, we show that active enzymes can be delivered into HEK293 cells in vitro when they are attached to tubular DNA origami nanostructures. We use bioluminescent enzymes as a cargo and monitor their activity from a cell lysate. The results show that the enzymes stay intact and retain their activity in the transfection process. The method is highly modular, which makes it a compelling candidate for a great variety of delivery applications.
