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Related Experiment Video

Updated: Mar 11, 2026

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration
06:22

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Published on: July 8, 2021

2.6K

Microstructure analysis method for evaluating degenerated intervertebral disc tissue.

Andrea Friedmann1, Felix Goehre2, Christopher Ludtka3

  • 1Fraunhofer Institute for Microstructure of Materials and Systems IMWS, Department of Biological and Macromolecular Materials, Halle, Germany.

Micron (Oxford, England : 1993)
|November 22, 2016
PubMed
Summary

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This study reveals microstructural changes in degenerated intervertebral disc (IVD) tissue using scanning electron microscopy (SEM). Understanding these changes is key for developing new therapies for intervertebral disc degeneration (IDD).

Area of Science:

  • Biomedical Engineering
  • Histopathology
  • Regenerative Medicine

Background:

  • Intervertebral disc (IVD) degeneration involves complex biomechanical, biochemical, and cellular interactions.
  • While cellular changes are understood, microstructural matrix deterioration in IVD degeneration remains under-explored.

Purpose of the Study:

  • To characterize microstructural and matrix changes in degenerated IVD tissue.
  • To evaluate the utility of scanning electron microscopy (SEM) for investigating intervertebral disc degeneration (IDD).

Main Methods:

  • Histology, light microscopy, and resin-embedded scanning electron microscopy (SEM).
  • Quantitative analysis of matrix delamination, cell density, cell size, and extracellular matrix (ECM) thickness.
  • Qualitative assessment of matrix fibration, neovascularization, and cell clustering.
Keywords:
Cell densityDelaminationDisc tissueIDDMicrostructureSEMScanning electron microscopy

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Related Experiment Videos

Last Updated: Mar 11, 2026

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration
06:22

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Published on: July 8, 2021

2.6K
An In Vitro Organ Culture Model of the Murine Intervertebral Disc
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An In Vitro Organ Culture Model of the Murine Intervertebral Disc

Published on: April 11, 2017

10.4K
Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture
13:37

Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture

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Main Results:

  • Corroborated known correlations: female gender with higher cell density and ECM thickness, and age with larger cell size.
  • Successfully quantified microstructural parameters of IDD using SEM.
  • Identified SEM's advantage in preserving tissue micromorphology compared to other methods.

Conclusions:

  • SEM is a valuable tool for detailed micromorphological analysis of degenerated IVD tissue.
  • Investigating microstructural changes enhances understanding of the complex IDD process.
  • A comprehensive understanding of IDD pathology is vital for advancing regenerative therapies.