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Protein Glycosylation

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...

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Updated: Mar 11, 2026

Author Spotlight: MAPP Protocol – Advancing Glycan Analysis

Author Spotlight: MAPP Protocol – Advancing Glycan Analysis

Published on: September 29, 2023

Clickable Polymeric Coating for Glycan Microarrays.

Caterina Zilio¹, Laura Sola², Marina Cretich¹

¹Istituto di Chimica del Riconoscimento Molecolare, CNR, Via Mario Bianco 9, 20131, Milan, Italy.

Methods in Molecular Biology (Clifton, N.J.)

|November 23, 2016

Summary

This summary is machine-generated.

This study introduces a new glycan microarray support for improved carbohydrate-protein interaction analysis. The novel surface enhances sensitivity and simplifies the immobilization of complex sugars using click chemistry.

Keywords:

Click chemistry Glycan microarray Oriented immobilization Polymeric coating

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Area of Science:

Carbohydrate chemistry
Biotechnology
Surface science

Background:

Carbohydrate interactions with biological targets are crucial in biology.
Glycan microarrays are essential tools for studying glycan-binding proteins.
Existing microarray technologies have limitations in sensitivity and fabrication.

Purpose of the Study:

To develop a novel microarray support for enhanced glycan array fabrication.
To combine high sensitivity with a versatile and easy-to-use immobilization method.
To facilitate the study of carbohydrate-protein interactions.

Main Methods:

Fabrication of a novel microarray support using a layered Si-SiO2 surface.
Application of a novel polymeric coating with alkyne functional groups from an aqueous solution.
Oriented immobilization of carbohydrates via azide/alkyne Cu (I) catalyzed "click" reaction.
Utilizing a functional 3D polymer coating for carbohydrate attachment.

Main Results:

The novel support provides a soft, tridimensional matrix for oriented carbohydrate immobilization.
The "click" chemistry approach allows for efficient and specific attachment of complex sugars.
The Si-SiO2 substrate ensures high fluorescence sensitivity and a superior signal-to-noise ratio.
The method simplifies glycan array fabrication, requiring no specialized personnel or labs.

Conclusions:

The developed glycan microarray support offers a sensitive, robust, and user-friendly platform for studying carbohydrate interactions.
This approach advances the field of glycomics by enabling accessible and efficient glycan array construction.
The technology has broad implications for understanding biological processes involving carbohydrates.