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DNA Microarrays02:34

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Label-Free Sensing on Microarrays.

Yung-Shin Sun1

  • 1Department of Physics, Fu-Jen Catholic University, New Taipei City, Taiwan, 24205. 089957@mail.fju.edu.tw.

Methods in Molecular Biology (Clifton, N.J.)
|November 23, 2016
PubMed
Summary
This summary is machine-generated.

Label-free biosensors in microarrays offer sensitive, real-time analysis of biomolecular interactions without altering molecule properties. This trend advances high-throughput screening for drug discovery and "-omics" research.

Keywords:
High-throughput screeningLabel-free biosensorMicroarrayOblique-incidence reflectivity difference (OI-RD)Surface plasmon resonance (SPR)

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Microarrays enable high-throughput screening of numerous biomolecular interactions simultaneously.
  • Traditional fluorescence-based detection methods require labeling, which can alter biomolecule properties and affect interactions.
  • Label-free biosensors are emerging as a superior alternative for analyzing biomolecular interactions.

Purpose of the Study:

  • To review principles and recent advancements in label-free sensing technologies for microarrays.
  • To highlight the advantages of label-free detection over traditional fluorescence methods.
  • To focus on surface plasmon resonance microscopy and oblique-incidence reflectivity difference microscopy.

Main Methods:

  • Development of various label-free sensing technologies for microarray applications.
  • Utilizing biosensors to monitor probe-target interactions in real-time.
  • Employing techniques such as surface plasmon resonance microscopy and oblique-incidence reflectivity difference microscopy.

Main Results:

  • Label-free biosensors provide comparable sensitivity to fluorescence methods.
  • These biosensors retain the conformational and functional integrity of biomolecules.
  • Real-time monitoring of binding affinities is achievable with certain label-free biosensors.

Conclusions:

  • Label-free biosensors are increasingly adopted in microarray platforms for their ability to provide accurate and sensitive detection.
  • They are crucial for advancing high-throughput screening in drug discovery and various fields of "-omics."
  • Continued development in label-free technologies promises further improvements in biomolecular interaction analysis.