Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains Using High Throughput...

Area of Science:

• Microbiology
• Genomics
• Epidemiology

Background:

• High-Throughput Sequencing (HTS) has revolutionized microbial typing and molecular epidemiology.
• HTS offers cost-effective analysis of draft genomes for various genetic determinants.

Purpose of the Study:

• To provide protocols for analyzing HTS data.
• To determine multilocus sequence typing (MLST) information from HTS data.
• To identify the presence or absence of antibiotic resistance genes using HTS data.

Main Methods:

• Analysis of High-Throughput Sequencing data.
• Determination of multilocus sequence typing (MLST) profiles.
• Identification of antibiotic resistance genes.

Main Results:

• Established protocols for HTS data analysis in microbial typing.
• Enabled determination of MLST and antibiotic resistance gene profiles from HTS data.

Conclusions:

• HTS is a powerful tool for microbial typing and molecular epidemiology.
• The provided protocols facilitate the use of HTS for identifying epidemiological markers and antimicrobial resistance.