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Related Experiment Video

Updated: Mar 11, 2026

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Published on: September 28, 2022

Affinity Purification-Mass Spectroscopy Methods for Identifying Epstein-Barr Virus-Host Interactions.

Anna A Georges¹, Lori Frappier²

¹Department of Molecular Genetics, University of Toronto, 1 Kings College Circle, Toronto, ON, Canada, M5S 1A8.

Methods in Molecular Biology (Clifton, N.J.)

|November 23, 2016

Summary

This study details methods for identifying viral protein interactions with host cells using affinity purification-mass spectrometry (AP-MS) and tandem affinity purification (TAP) tagging. These techniques enable comprehensive discovery of Epstein-Barr virus (EBV) protein interactions.

Keywords:

Affinity purification Epstein–Barr virus FLAG tag Mass spectrometry Protein interactions Proteomics SPA tag TAP tag

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Last Updated: Mar 11, 2026

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Area of Science:

Virology
Molecular Biology
Proteomics

Background:

Understanding viral protein function requires identifying their cellular interaction partners.
Mass spectrometry-based proteomics offers comprehensive and unbiased discovery of protein interactions.
Previous studies utilized AP-MS and TAP tagging for Epstein-Barr virus (EBV) protein interaction discovery, but applied them to limited EBV proteins.

Purpose of the Study:

To provide detailed methodologies for AP-MS and TAP tagging.
To enable the application of these methods to any EBV protein for host interaction discovery.

Main Methods:

Affinity purification coupled with mass spectrometry (AP-MS) for identifying protein complexes.
Tandem affinity purification (TAP) tagging for robust isolation and identification of protein interactions.
Application of these techniques to Epstein-Barr virus (EBV) proteins.

Main Results:

Detailed protocols for AP-MS and TAP tagging are presented.
These methods are adaptable for investigating interactions of any EBV protein.
The study facilitates broader discovery of EBV-host protein interactions.

Conclusions:

AP-MS and TAP tagging are powerful, versatile methods for EBV-host interaction discovery.
Standardized protocols will accelerate the identification of novel viral-cellular interactions.
This work provides a foundation for understanding EBV pathogenesis through protein interaction networks.