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Engineering Plant Immunity: Using CRISPR/Cas9 to Generate Virus Resistance
Syed Shan-E-Ali Zaidi1, Manal Tashkandi2, Shahid Mansoor3
1Laboratory for Genome Engineering, Division of Biological Sciences, King Abdullah University of Science and TechnologyThuwal, Saudi Arabia; National Institute for Biotechnology and Genetic EngineeringFaisalabad, Pakistan.
Frontiers in Plant Science
|November 24, 2016
Summary
The CRISPR/Cas9 genome-editing tool offers a powerful new strategy to combat plant viruses threatening global food security. This technology can engineer virus resistance in crops, protecting essential agricultural yields.
Area of Science:
- Agricultural Science
- Molecular Biology
- Plant Pathology
Background:
- Plant viruses endanger global food security by threatening vital crops like wheat, maize, and cotton.
- Conventional control methods are insufficient against rapidly evolving and emerging plant viruses.
Approach:
- Utilizes the CRISPR (clustered regularly interspaced palindromic repeats)/CRISPR-associated 9 (CRISPR/Cas9) system, a precise genome-engineering technology.
- Applies CRISPR/Cas9 to directly target viral genomes or enhance plant immunity through host genome modification.
Key Points:
- CRISPR/Cas9 demonstrates simplicity, efficiency, and reproducibility in engineering virus resistance.
- Studies show successful viral interference by directly cleaving viral DNA or modifying plant genomes.
- This technology holds promise for improving crop yields and global agricultural sustainability.
Conclusions:
- CRISPR/Cas9-mediated viral immunity presents a significant advancement for crop protection.
- Further research is needed to address knowledge gaps and outstanding questions for widespread application.
- This technology can enhance global agriculture by safeguarding crops against viral threats.
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