Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extracellular vesicles in nipple discharge for breast cancer screening.

Breast cancer research and treatment·2026
Same author

Dual-readout villus-like scaffold microfluidic platform for quantitative analysis of gut microbiota formation under perfusion.

The Analyst·2026
Same author

A microfluidic dermal fibroblast-macrophage co-culture on a chip linking inflammatory signalling to barrier-associated function.

Lab on a chip·2026
Same author

DNA Copy Number Profiling in Extracellular Vesicles as Clinical Biomarkers of High-Grade Serous Ovarian Carcinoma.

Journal of extracellular vesicles·2026
Same author

Successful Surgical Treatment of Pediatric Intestinal Behçet's Disease with Ileocecal Ulcer Refractory to Medical Therapy: A Case Report.

Surgical case reports·2026
Same author

[Pharmacists as Gatekeepers for Overdose and Suicide Prevention: Providing a Good Background on the Current Situation and Evidence].

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2026

Related Experiment Video

Updated: Mar 11, 2026

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

3.2K

Identifying DNA methylation in a nanochannel.

Xiaoyin Sun1, Takao Yasui2, Takeshi Yanagida3

  • 1Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya, Japan; ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Nagoya, Japan.

Science and Technology of Advanced Materials
|November 24, 2016
PubMed
Summary

This study presents a rapid method to detect DNA methylation, a key epigenetic marker, without protein modifications. The new technique analyzes single DNA molecules in nanochannels, offering results in just 2 hours.

Keywords:
102 Porous / Nanoporous / Nanostructured materials30 Bio-inspired and biomedical materialsDNA contractionDNA methylationnanochannelsingle DNA molecule

More Related Videos

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
11:13

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

11.3K
Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

14.2K

Related Experiment Videos

Last Updated: Mar 11, 2026

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

3.2K
Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
11:13

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

11.3K
Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

14.2K

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Biophysics

Background:

  • DNA methylation is a crucial epigenetic modification regulating gene expression.
  • Current DNA methylation analysis methods are time-consuming, requiring 24-96 hours.
  • Existing methods often involve DNA replication and protein modification.

Purpose of the Study:

  • To develop a faster method for analyzing DNA methylation.
  • To enable DNA methylation detection at the single DNA molecule level.
  • To avoid protein modifications in the analysis process.

Main Methods:

  • Measuring contracted DNA length and relaxation time within a nanochannel.
  • Utilizing the increased stiffness of methylated DNA.
  • Analyzing single DNA molecules without protein modification.

Main Results:

  • Methylated DNA exhibits a longer contracted length in nanochannels.
  • Methylated DNA shows a longer relaxation time, indicating slower contraction.
  • The methodology successfully detects DNA methylation at the single-molecule level.

Conclusions:

  • This novel methodology provides a rapid (within 2 hours) way to analyze DNA methylation.
  • The technique allows for single DNA molecule analysis without protein modification.
  • This approach offers a promising alternative to conventional time-consuming methods.