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Updated: Mar 11, 2026

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
Published on: November 13, 2021
Sequence protein identification by randomized sequence database and transcriptome mass spectrometry (SPIDER-TMS):
Raffaella Pascale1, Gerarda Grossi2, Gabriele Cruciani3
1Scuola di Ingegneria, Università degli Studi della Basilicata, Via dell'Ateneo Lucano, 10-85100 Potenza, Italy.
Abstract:
Sequence protein identification by a randomized sequence database and transcriptome mass spectrometry software package has been developed at the University of Basilicata in Potenza (Italy) and designed to facilitate the determination of the amino acid sequence of a peptide as well as an unequivocal identification of proteins in a high-throughput manner with enormous advantages of time, economical resource and expertise. The software package is a valid tool for the automation of a de novo sequencing approach, overcoming the main limits and a versatile platform useful in the proteomic field for an unequivocal identification of proteins, starting from tandem mass spectrometry data. The strength of this software is that it is a user-friendly and non-statistical approach, so protein identification can be considered unambiguous.
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