Pulmonary carcinogenesis in rats given implants of cigarette smoke condensate in beeswax pellets

Lungs of inbred OM/NCR and outbred Sprague-Dawley rats were given implants, through a thoracotomy, of pellets of cigarette smoke condensate (CSC) suspended in a beeswax-tricaprylin vehicle. The pellets slowly released material into the surrounding parenchyma, which resulted in a dose-related increased incidence of lung cancer, predominantly invasive and metastasizing epidermoid carcinoma. A 42% prevalence of pulmonary carcinoma was present in the highest dosage group, which received 67 mg CSC, exposing approximately 1.65 cm2 bronchiolar epithelium. Squamous metaplasia associated with the implanted site preceded the appearance of the carcinomas and was more severe, with the larger pellets having more concentrated CSC. No difference was observed in incidence of pulmonary carcinomas with the use of CSC containing high or low concentrations of nicotine. The potential value of this bioassay system were discussed.