Related Experiment Video
Updated: Mar 11, 2026
08:30
Glaucoma-inducing Procedure in an In Vivo Rat Model and Whole-mount Retina Preparation
Published on: March 12, 2016
13.7K
Relevant variations and neuroprotecive effect of hydrogen sulfide in a rat glaucoma model
Shouyue Huang1, Ping Huang2, Xiaohong Liu1
1Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, 197 Ruijin Er Road, 200025 Shanghai, China.
Neuroscience
|November 29, 2016
Summary
Hydrogen sulfide (H2S) levels decrease in experimental glaucoma, contributing to retinal ganglion cell (RGC) loss. Supplementing H2S protects RGCs, suggesting a therapeutic role in glaucoma.
Area of Science:
- Ophthalmology
- Neuroscience
- Biochemistry
Background:
- Glaucoma is an irreversible neurodegenerative disease causing blindness through retinal ganglion cell (RGC) loss.
- Endogenous hydrogen sulfide (H2S) is implicated in central nervous system neurodegeneration.
Purpose of the Study:
- To develop a chronic ocular hypertension (COH) rat model for glaucoma research.
- To investigate H2S levels and related enzyme expression in experimental glaucoma.
- To evaluate the neuroprotective effect of exogenous H2S on RGCs.
Main Methods:
- A COH rat model was induced via anterior chamber hydrogel injection.
- Intraocular pressure (IOP), RGC counts, and retinal morphology were assessed.
- Retinal H2S levels and synthases (CBS, CSE, 3-MST) expression were measured.
- The effect of NaHS (H2S donor) on RGC survival was evaluated.
Main Results:
- The COH model successfully mimicked glaucoma features.
- Retinal H2S levels and expression of CBS, CSE, and 3-MST were significantly reduced in COH rats.
- NaHS treatment improved RGC survival and restored H2S levels in COH rats without altering IOP.
Conclusions:
- Intracameral hydrogel injection is effective for modeling glaucoma.
- Reduced H2S is involved in glaucomatous neuropathy.
- Exogenous H2S demonstrates a protective effect on RGCs in experimental glaucoma.
