Related Experiment Videos
A cytotoxic serine proteinase isolated from mouse submandibular gland
T Shimamura1, N Nagumo, H Ikigai
1Department of Bacteriology and Immunology, Showa University School of Medicine, Tokyo, Japan.
Immunology Letters
|August 1, 1989
Abstract:
We have isolated a novel cytotoxic factor from the submandibular glands of male BALB/c mice by Sephadex G-50 gel filtration chromatography and reverse-phase HPLC. The cytotoxic factor is a serine proteinase, which belongs to the mouse glandular kallikrein (mGK) family, with an Mr of approximately 27,000. The purified serine proteinase showed cytotoxic activity against mouse thymocytes in a dose-dependent manner, and a serine proteinase inhibitor, diisopropyl fluorophosphate, blocked its cytotoxic activity.