Identification and expression analyses of vitellogenin in Bactericera cockerelli (Šulc)

The potato psyllid, Bactericera cockerelli (Šulc) (Hemiptera: Triozidae), is a phloem-feeding insect with preference for Solanaceae. This insect species is vector of the pathogenic bacteria 'Candidatus Liberibacter solanacearum' the causative agent of zebra chip, an important disease of commercial potatoes in several countries worldwide. The recent classification of psyllids among the most dangerous vectors has promoted their study, but still many biological processes such as reproduction and vitellogenesis need to be investigated. As a first step towards the elucidation of vitellogenesis in B. cockerelli, one candidate vitellogenin transcript (6622 bases long) was identified and the expression of the transcript and the protein were analyzed in virgin and mated females between 1 and 7days old. In virgin females, Vg expression increased up to 5days old; while mating significantly up-regulated Vg expression in 5- and 7-day-old females. To determine the role of juvenile hormone in B. cockerelli Vg expression, topical applications of juvenile hormone III were performed on virgin females, resulting in an up-regulation of Vg expression and an increase in the number of mature oocytes present in female reproductive organs. Overall, this study represents the first step to understand vitellogenesis of B. cockerelli and it highlights the role of JH III in the hormonal regulation of Vg expression and oocyte development.