Related Experiment Videos
Complete nucleotide sequence of the matrix protein mRNA of mumps virus
1Department of Virology, School of Medicine, Karolinska Institute, Stockholm, Sweden.
Abstract:
The complete nucleotide sequence of the mumps virus membrane protein or matrix protein (M) has been determined by sequencing cDNA clones and confirmed by partially sequencing the M mRNA and the genome. The mRNA is 1248 nucleotides long excluding the poly(A) and encodes a protein of 375 amino acids. The molecular weight (38,670), deduced from the amino acid sequence, is in agreement with the molecular weight of the viral M protein estimated by polyacrylamide gel electrophoresis (39-40 kDa). The mumps virus M protein shows 23-27% homology with M proteins of Newcastle disease virus (NDV), measles virus, canine distemper virus (CDV), parainfluenza virus type 3, and Sendai virus, respectively. A comparison of the M protein sequences of the above six paramyxoviruses did not reveal any conserved area of homology common among all paramyxovirus M proteins.
Insights
The complete nucleotide sequence of the mumps virus matrix (M) protein was determined. This viral protein shares 23-27% homology with related paramyxoviruses but lacks a conserved region across all of them.
Area of Science:
- Virology
- Molecular Biology
- Genomics
Background:
- The mumps virus is a significant human pathogen.
- Understanding viral protein structure and function is crucial for developing antiviral strategies.
- The matrix (M) protein plays a key role in paramyxovirus assembly and budding.
Purpose of the Study:
- To determine the complete nucleotide sequence of the mumps virus matrix (M) protein.
- To analyze the deduced amino acid sequence and compare it with M proteins of other paramyxoviruses.
Main Methods:
- Sequencing of complementary DNA (cDNA) clones derived from mumps virus mRNA.
- Partial sequencing of the M mRNA and the viral genome.
- Deduction of amino acid sequence and molecular weight.
- Comparison of amino acid sequences with homologous proteins from other paramyxoviruses using sequence alignment.
Main Results:
- The complete nucleotide sequence of the mumps virus M protein mRNA was determined to be 1248 nucleotides.
- The M protein consists of 375 amino acids with a deduced molecular weight of 38,670 Da, consistent with experimental estimations (39-40 kDa).
- Mumps virus M protein exhibits 23-27% homology with M proteins of Newcastle disease virus, measles virus, canine distemper virus, parainfluenza virus type 3, and Sendai virus.
Conclusions:
- The nucleotide and amino acid sequence of the mumps virus M protein have been elucidated.
- While showing homology with other paramyxovirus M proteins, no conserved region common to all six paramyxoviruses was identified.
- This detailed sequence information provides a basis for further functional and evolutionary studies of paramyxovirus M proteins.