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Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
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Using electron microscopy to calculate optical properties of biological samples
Wenli Wu1, Andrew J Radosevich2, Adam Eshein2
1Applied Physics Program, Northwestern University, Evanston, Illinois 60208, USA.
Biomedical Optics Express
|November 30, 2016
Summary
We developed a new electron microscopy (EM) method to determine optical properties of biological structures. This technique accurately calculates optical properties for human colon cell nuclei, validating its effectiveness.
Area of Science:
- Biophysics
- Optical Physics
- Cell Biology
Background:
- The relationship between microscopic structure and optical properties in biological tissues is not fully understood.
- Improved understanding can enhance current optical techniques and enable new discoveries.
Purpose of the Study:
- To introduce a novel electron microscopy (EM) based method for calculating optical properties of biological structures.
- To demonstrate the technique using human epithelial colon cell nuclei and validate its accuracy.
Main Methods:
- Utilized electron microscopy (EM) to obtain high-resolution images of biological samples.
- Developed an analysis technique to compute optical properties from EM data.
- Calculated the anisotropy factor (g), phase function, and shape factor (D) for cell nuclei.
Main Results:
- Successfully calculated the spectrum of anisotropy factor g, phase function, and shape factor D for human colon cell nuclei.
- Demonstrated strong agreement between calculated optical properties and an independent study.
- Validated the novel EM-based method for optical property measurement.
Conclusions:
- The proposed EM-based method offers a new approach to extract the intrinsic phase function of biological samples.
- This technique provides an independent validation for existing optical property measurement methods.
- Advances understanding of structure-optical property relationships in biological media.
Keywords:
(000.1430) Biology and medicine(160.4760) Optical properties(170.3660) Light propagation in tissues(170.4580) Optical diagnostics for medicineMore Related Videos
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