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A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Wide-field Fourier ptychographic microscopy using laser illumination source.

Jaebum Chung¹, Hangwen Lu¹, Xiaoze Ou¹

¹Department of Electrical Engineering, California Institute of Technology, Pasadena, California, 91125, USA.

Biomedical Optics Express

|November 30, 2016

Summary

This study introduces a guided laser illumination for Fourier ptychographic (FP) microscopy, enhancing acquisition speed and achieving a 4x resolution improvement. Speckle artifacts were mitigated using differential phase contrast (DPC) deconvolution.

Keywords:

(070.0070) Fourier optics and signal processing (110.1758) Computational imaging (180.0180) Microscopy

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Area of Science:

Microscopy
Optical Imaging
Coherent Imaging

Background:

Fourier ptychographic (FP) microscopy synthesizes high-bandwidth images from multiple low-bandwidth inputs.
FP microscopy requires bright illumination and high-speed cameras for efficient data acquisition.
Laser illumination in FP microscopy can introduce speckle artifacts.

Purpose of the Study:

To implement a guided laser beam as an illumination source for FP microscopy.
To improve the speed and resolution of FP imaging.
To mitigate speckle artifacts in laser-illuminated FP microscopy.

Main Methods:

Utilized a guided laser beam with a mirror array and Galvo scanning system for diverse plane-wave illuminations.
Integrated differential phase contrast (DPC) deconvolution to obtain phase images for artifact reduction.
Employed a 1-Watt laser providing 150 mW collimated beam power for rapid image capture.

Main Results:

Achieved a total capture time of 0.96 seconds for 96 raw FP images, limited by camera frame rate.
Demonstrated a 4x resolution enhancement over the 2.7 mm x 1.5 mm field-of-view with a 0.1 NA objective lens.
Successfully mitigated speckle artifacts by incorporating DPC-derived phase information into the FP reconstruction algorithm.

Conclusions:

A guided laser illumination system is effective for accelerating FP microscopy.
The combined FP and DPC approach successfully reduces speckle noise, improving image quality.
This method significantly enhances resolution and imaging speed in FP microscopy.