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Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
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Data for increased Rho kinase activity in type 2 diabetic patients
Lei Liu1, Lun Tan1, Jinsheng Lai1
1Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Data in Brief
|November 30, 2016
Summary
Rho-Kinase (ROCK) activity is elevated in type 2 diabetes patients, suggesting its role in the disease. Measuring ROCK activity and IL-6 levels offers insights into diabetic pathophysiology.
Area of Science:
- Biochemistry
- Cardiovascular Research
- Endocrinology
Background:
- Rho-Kinase (ROCK) is a key regulator of cellular processes and a potential therapeutic target for cardiovascular diseases.
- Type 2 diabetes is associated with significant cardiovascular complications.
- Understanding the role of ROCK in diabetes is crucial for developing targeted therapies.
Purpose of the Study:
- To investigate the relationship between Rho-Kinase activity and type 2 diabetes.
- To quantify Rho-Kinase activity in peripheral blood leukocytes of type 2 diabetic patients.
Main Methods:
- Rho-Kinase activity was assessed by immunoblotting peripheral blood leukocytes.
- Specific measurement involved detecting Phospho-Thr853 in the myosin-binding subunit of myosin light-chain phosphatase.
- Interleukin-6 (IL-6) levels were quantified using Enzyme-linked immunosorbent assay (ELISA).
Main Results:
- Data indicate an observed relationship between elevated Rho-Kinase activity and type 2 diabetic patients.
- IL-6 levels were measured as a potential biomarker in conjunction with ROCK activity.
Conclusions:
- Enhanced Rho-Kinase activity is linked to type 2 diabetes.
- These findings highlight ROCK as a relevant factor in the pathophysiology of type 2 diabetes.
- Further research into ROCK inhibition may offer therapeutic strategies for diabetic complications.
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