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Updated: Mar 11, 2026

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

Studying Antibody Repertoires with Next-Generation Sequencing.

William D Lees¹, Adrian J Shepherd²

¹Department of Biological Sciences and Institute of Structural and Molecular Biology, Birkbeck, University of London, Malet Street, London, WC1E 7HX, UK.

Methods in Molecular Biology (Clifton, N.J.)

|November 30, 2016

Summary

Next-generation sequencing (NGS) reveals antibody repertoire details for studying infections, autoimmunity, and cancer. Challenges in NGS methods require careful experimental design for antibody repertoire analysis.

Keywords:

Antibodies Antibodyome Next generation sequencing Rep-Seq Repertoire analysis

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Last Updated: Mar 11, 2026

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Published on: March 15, 2019

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Published on: August 22, 2019

Area of Science:

Immunology
Genomics
Bioinformatics

Background:

Next-generation sequencing (NGS) enables detailed study of antibody repertoires.
Antibody repertoire analysis is crucial for understanding immune responses in infection, autoimmunity, and cancer.

Purpose of the Study:

To outline contexts for antibody repertoire analysis.
To summarize available tools for repertoire studies.
To review methods and conclusions from vaccination studies.

Main Methods:

Utilizing next-generation sequencing (NGS) for high-throughput antibody repertoire analysis.
Addressing challenges posed by repertoire polymorphism in experimental design.
Reviewing established tools and methodologies for repertoire characterization.

Main Results:

NGS provides unprecedented detail on antibody repertoires.
Polymorphism in antibody repertoires presents unique analytical challenges.
Vaccination studies have significantly advanced repertoire analysis.

Conclusions:

Antibody repertoire analysis is a powerful tool for studying immune responses.
Careful consideration of NGS methods and experimental design is vital.
Further research in this area can yield insights into disease mechanisms and vaccine efficacy.