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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Mar 11, 2026

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Multiplex Analysis Using cDNA Transcriptomic Profiling.

Steve F C Hawkins¹, Paul C Guest²

¹Bioline Reagents Limited, Unit 16, The Edge Business Centre, Humber Road, London, NW2 6EW, UK. shawkins@bioline.com.

Methods in Molecular Biology (Clifton, N.J.)

|November 30, 2016

Summary

This summary is machine-generated.

This study introduces a high-density complementary DNA (cDNA) microarray method to track messenger RNA (mRNA) changes during neuronal precursor cell differentiation. The technique allows for precise quantification of gene expression during this crucial developmental process.

Keywords:

Fluorescent dyes Multiplex analysis Quantitation Taq polymerase cDNA mRNA

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Area of Science:

Molecular Biology
Neuroscience
Genomics

Background:

DNA microarrays enable the simultaneous measurement of thousands of gene expression levels.
Understanding gene regulation during cell differentiation is crucial for developmental biology and regenerative medicine.

Purpose of the Study:

To present a method for detecting mRNA transcriptional changes in neuronal precursor cells during differentiation.
To utilize high-density complementary DNA (cDNA) microarrays for this analysis.

Main Methods:

Utilized high-density cDNA microarrays.
Detected and quantitated hybridization of fluorescently labeled target DNA to specific DNA probe sequences.
Focused on mRNA transcriptional changes in neuronal precursor cells.

Main Results:

Successfully detected mRNA transcriptional changes.
Quantified gene expression alterations during neuronal differentiation.
Demonstrated the utility of high-density cDNA microarrays in this context.

Conclusions:

The presented method is effective for analyzing gene expression dynamics during neuronal differentiation.
High-density cDNA microarrays provide a powerful tool for studying transcriptional regulation in cellular development.