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Lab-on-a-Chip Multiplex Assays.

Harald Peter1, Julia Wienke2, Frank F Bier2

  • 1Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (IZI-BB), Am Mühlenberg 13, 14476, Potsdam, Germany. Harald.Peter@izi-bb.fraunhofer.de.

Methods in Molecular Biology (Clifton, N.J.)
|November 30, 2016
PubMed
Summary
This summary is machine-generated.

This study presents a rapid, automated DNA microarray assay for identifying bacterial species and antibiotic resistance genes in under 10 minutes. The Fraunhofer in vitro diagnostics (ivD) platform enables quick adaptation for new biomarkers like Staphylococcus aureus and the mecA resistance gene.

Keywords:
Antibiotic resistance detectionAntimicrobial drug resistanceAutomated DNA microarray hybridizationBacterial infectionEarly diagnosisGenotypingLab-on-a-chipMRSA detectionPoint-of-careSNP detection

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Area of Science:

  • * Molecular Diagnostics
  • * Microbiology
  • * Biotechnology

Background:

  • * Lab-on-a-chip multiplex assays offer automated, rapid identification of multiple parameters.
  • * DNA microarrays facilitate swift adaptation of new biomarkers for gene and single-nucleotide-polymorphism (SNP) detection.

Purpose of the Study:

  • * To describe a rapid, fully automated DNA microarray hybridization and readout protocol.
  • * To enable quick identification of bacterial species and antibiotic resistance.
  • * To present a DNA microarray for detecting Staphylococcus aureus and the mecA resistance gene.

Main Methods:

  • * Development of a lab-based preparation protocol.
  • * Implementation of a Fraunhofer in vitro diagnostics (ivD) platform for automated assay execution.
  • * DNA microarray hybridization and readout in under 10 minutes.

Main Results:

  • * Achieved rapid and fully automated DNA microarray hybridization and readout.
  • * Demonstrated successful identification of bacterial species and antibiotic resistance genes.
  • * Developed a specific DNA microarray for Staphylococcus aureus and mecA gene detection.

Conclusions:

  • * The described protocol enables rapid bacterial identification and antibiotic resistance detection using DNA microarrays.
  • * The Fraunhofer ivD platform facilitates fast assay adaptation for emerging diagnostic needs.
  • * This approach is valuable for timely clinical and research applications in microbiology.