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Updated: Mar 11, 2026
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Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
Published on: July 4, 2016
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Noncovalent and site-directed spin labeling of duplex RNA
Nilesh R Kamble1, Markus Gränz2, Thomas F Prisner2
1Department of Chemistry, Science Institute, University of Iceland, Dunhaga 3, 107 Reykjavik, Iceland. snorrisi@hi.is.
Chemical Communications (Cambridge, England)
|December 1, 2016
Abstract:
An isoindoline-nitroxide derivative of guanine (Ǵ, "G-spin") was shown to bind specifically and effectively to abasic sites in duplex RNAs. Distance measurements on a Ǵ-labeled duplex RNA with PELDOR (DEER) showed a strong orientation dependence. Thus, Ǵ is a readily synthesized, orientation-selective spin label for "mix and measure" PELDOR experiments.
