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Updated: Mar 11, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

An Assemblable, Multi-Angle Fluorescence and Ellipsometric Microscope.

Victoria Nguyen¹, John Rizzo², Babak Sanii^1,2,3

¹Keck Science Department, Scripps College, Claremont, CA, United States of America.

|December 2, 2016

Summary

This summary is machine-generated.

Researchers developed a low-cost, multi-functional microscope adaptable to various geometries and imaging techniques. This open-source design is easily assembled, making advanced microscopy accessible for labs with space and budget constraints.

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Area of Science:

Optical microscopy
Biophysics
Materials science

Background:

Research laboratories often face significant cost and space limitations for advanced microscopy.
Existing microscopes may lack versatility in imaging geometries and techniques.
Developing affordable, adaptable instrumentation is crucial for broader scientific accessibility.

Purpose of the Study:

To introduce a novel, multi-functional microscope addressing cost and space constraints in research laboratories.
To present an open-source design for a versatile microscope capable of multiple imaging modes and geometries.
To demonstrate the utility of this microscope for studying hydrated lipid films and biological samples.

Main Methods:

The microscope design pivots around the sample, enabling upright, inverted, side-on, and oblique geometries.
It integrates bright-field, fluorescence, and qualitative ellipsometric imaging capabilities.
The system is assembled from off-the-shelf and 3D-printed components, with a detailed manual provided.

Main Results:

The developed microscope is the first single instrument to perform all introduced functionalities.
Assembly by two undergraduate students is feasible in under a day, costing approximately $16k (2016 prices).
The open design approach allows for customization and adaptation to specific research needs.

Conclusions:

The multi-functional, adaptable microscope offers a cost-effective solution for laboratories with limited resources.
The open-source design promotes accessibility and customization in advanced optical microscopy.
The instrument is demonstrated as effective for studying hydrated lipid films and wet biological samples.