Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme Inhibition01:30

Enzyme Inhibition

94.3K
Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
94.3K
Feedback Inhibition00:46

Feedback Inhibition

58.2K
Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
58.2K
The Electron Transport Chain01:30

The Electron Transport Chain

20.8K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
20.8K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

6.1K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.1K
Riboswitches01:56

Riboswitches

10.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
10.0K
Structural Protein Function01:56

Structural Protein Function

30.3K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
30.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The intracellular inositol (pyro)phosphate receptor AtSPX1 reciprocally binds to P1BS DNA.

Nature communications·2026
Same author

A minimum catalytic unit for synthesis of InsP6 and 5-PP-InsP5 in Arabidopsis.

The Biochemical journal·2025
Same author

Phosphorus-specific, liquid chromatography inductively coupled plasma mass spectrometry for analysis of inositol phosphate and inositol pyrophosphate metabolism.

The Biochemical journal·2025
Same author

Substrate promiscuity of inositol 1,4,5-trisphosphate kinase driven by structurally-modified ligands and active site plasticity.

Nature communications·2024
Same author

Crystal Structure and Enzymology of <i>Solanum tuberosum</i> Inositol Tris/Tetrakisphosphate Kinase 1 (<i>St</i>ITPK1).

Biochemistry·2023
Same author

2-Methoxyestradiol-3,17-<i>O</i>,<i>O</i>-bis-sulfamate (STX140) Inhibits Proliferation and Invasion via Senescence Pathway Induction in Human BRAFi-Resistant Melanoma Cells.

International journal of molecular sciences·2023
Same journal

Reaction Optimization for Enzymatic Deconstruction of Industrially Relevant Nylon Composites.

Chembiochem : a European journal of chemical biology·2026
Same journal

Deploying Artificial Metalloenzymes in Complex Environments: Strategies and Applications.

Chembiochem : a European journal of chemical biology·2026
Same journal

Synthetic Ligands of Myeloid C-Type Lectin Receptors.

Chembiochem : a European journal of chemical biology·2026
Same journal

Vancomycin-Mediated Binding of DNA Origami Nanostructures to Gram-Positive and Gram-Negative Bacteria.

Chembiochem : a European journal of chemical biology·2026
Same journal

Mutasynthesis and Antibiotic Activity of Mupirocin Analogues.

Chembiochem : a European journal of chemical biology·2026
Same journal

Pressure-Dependent Aromatic Ring Flips Reveal Variable Transition-State Volume and Compressibility Among Structural Regions of BPTI.

Chembiochem : a European journal of chemical biology·2026
See all related articles

Related Experiment Video

Updated: Mar 11, 2026

Evaluating Therapeutic Interventions in the SHIP-deficient Mouse Model of Crohn Disease-like Ileitis and Fibrosis
09:44

Evaluating Therapeutic Interventions in the SHIP-deficient Mouse Model of Crohn Disease-like Ileitis and Fibrosis

Published on: October 14, 2025

563

SHIP2: Structure, Function and Inhibition.

Mark P Thomas1, Christophe Erneux2, Barry V L Potter3

  • 1Department of Pharmacy and Pharmacology, University of Bath, Claverton Down, Bath, BA2 7AY, UK.

Chembiochem : a European Journal of Chemical Biology
|December 2, 2016
PubMed
Summary
This summary is machine-generated.

SHIP2, a phosphatase regulating phosphoinositides, is implicated in bone development and insulin signaling. Inhibitors targeting SHIP2 show in vivo effects, highlighting its therapeutic potential.

Keywords:
enzymesinhibitorsphosphatasesphospholipidsstructure

More Related Videos

Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors
08:45

Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors

Published on: July 17, 2020

6.7K
Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
10:08

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Published on: December 9, 2022

2.7K

Related Experiment Videos

Last Updated: Mar 11, 2026

Evaluating Therapeutic Interventions in the SHIP-deficient Mouse Model of Crohn Disease-like Ileitis and Fibrosis
09:44

Evaluating Therapeutic Interventions in the SHIP-deficient Mouse Model of Crohn Disease-like Ileitis and Fibrosis

Published on: October 14, 2025

563
Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors
08:45

Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors

Published on: July 17, 2020

6.7K
Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
10:08

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Published on: December 9, 2022

2.7K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • SHIP2 is a 5-phosphatase enzyme crucial for phosphoinositide metabolism.
  • It plays a role in opsismodysplasia, a bone development disorder.
  • SHIP2 interacts with proteins involved in insulin signaling, cytoskeletal dynamics, and immune function.

Purpose of the Study:

  • To review the known functions and structural information of SHIP2.
  • To discuss the development and efficacy of SHIP2 inhibitors.
  • To identify knowledge gaps and future research directions for SHIP2.

Main Methods:

  • Literature review of SHIP2 research.
  • Analysis of structural data for SHIP2 domains.
  • Evaluation of studies on SHIP2 inhibitors and their in vivo effects.

Main Results:

  • The structure of three SHIP2 domains is known.
  • Sub-micromolar IC50 inhibitors targeting the catalytic domain have been developed.
  • These inhibitors demonstrate in vivo effects consistent with SHIP2 inhibition.

Conclusions:

  • SHIP2 is a significant therapeutic target with potential applications in various diseases.
  • Further research is needed to fully elucidate SHIP2's diverse roles.
  • Development of SHIP2 inhibitors offers promising therapeutic avenues.