[Chromogens in systems for immunoenzyme determination using horseradish peroxidase-labeled antibodies]

The author tested the suitability of various chromogens in ELISA systems with horseradish peroxidase. As a model he used assessment of human chorionic gonadotropin (HCG). The author evaluated the influence of substrate concentration on the resulting colour reaction and the influence of the presence of a detergent on the peroxidase activity. He investigated also the advantages of the used chromogens with regard to the accuracy and sensitivity of the estimation.