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The Mouse Isolated Perfused Kidney Technique

The Mouse Isolated Perfused Kidney Technique

Published on: November 17, 2016

The Mouse Isolated Perfused Kidney Technique.

Jan Czogalla¹, Frank Schweda², Johannes Loffing³

¹Institute of Anatomy, Swiss National Centre of Competence in Research Kidney, University of Zürich; jan.czogalla@uzh.ch.

Journal of Visualized Experiments : Jove

|December 3, 2016

Summary

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The mouse isolated perfused kidney (MIPK) technique enables ex vivo organ study and future applications like bioengineering. This paper details the MIPK method and validates its success through functional, morphological, and molecular assessments.

Area of Science:

Physiology
Bioengineering
Transplantation

Background:

The mouse isolated perfused kidney (MIPK) is a critical ex vivo model.
It allows for organ manipulation, functional assessment, and pharmaceutical administration.
MIPK is a prerequisite for advanced applications like kidney bioengineering and pre-transplant therapies.

Purpose of the Study:

To describe the perfusate and surgical technique for mouse kidney ex vivo perfusion.
To present data validating the viability and functionality of the MIPK preparation.
To establish MIPK as a robust platform for kidney research.

Main Methods:

Detailed description of the perfusate composition and surgical procedures for MIPK.
Utilized a perfusion apparatus for maintaining kidney viability ex vivo.

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Last Updated: Mar 11, 2026

The Mouse Isolated Perfused Kidney Technique

The Mouse Isolated Perfused Kidney Technique

Published on: November 17, 2016

Optical Clearing and Imaging of Immunolabeled Kidney Tissue

Optical Clearing and Imaging of Immunolabeled Kidney Tissue

Published on: July 22, 2019

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Published on: January 18, 2019

Assessed kidney viability through functional, morphological, and molecular analyses.

Main Results:

Presented data on renal blood flow, vascular resistance, and urine output to demonstrate functional viability.
Included transmission electron micrographs of nephron segments for morphological assessment.
Showcased western blots of transport proteins as a molecular readout of kidney integrity.

Conclusions:

The described MIPK technique provides a viable and functional ex vivo model of the mouse kidney.
This method supports future innovative applications in kidney bioengineering and transplantation research.
MIPK serves as a valuable tool for studying kidney physiology and testing therapeutic interventions.