Related Experiment Video
Updated: Mar 10, 2026
10:41
Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
Published on: April 5, 2019
19.1K
Multidimensional liquid-phase separations combining both chromatography and electrophoresis - A review
Leila Ranjbar1, Joe P Foley2, Michael C Breadmore1
1Australian Centre for Research on Separation Science, School of Physical Sciences, University of Tasmania, Private Bag 75, Hobart, Tasmania 7001, Australia.
Analytica Chimica Acta
|December 6, 2016
Summary
Combining chromatography and electrophoresis (LC-E and E-LC) offers powerful two-dimensional separations for complex samples. This review details these hyphenated techniques for enhanced molecular resolution.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- The coupling of chromatography and electrophoresis (LC-E and E-LC) is a powerful technique for two-dimensional separations.
- These hyphenated systems enhance the resolution of diverse molecules in biological, environmental, and food samples.
Approach:
- This review comprehensively surveys multidimensional chromato-electrophoretic (LC-E) and electrophero-chromatographic (E-LC) systems.
- It covers separation modes including reversed-phase, ion-exchange, size-exclusion chromatography, and various electrophoresis techniques.
- The review discusses non-gel based off-line and on-line hyphenation technologies, detailing their advantages, disadvantages, conditions, and system characteristics.
Key Points:
- Explores the evolution of LC-E and E-LC systems from their inception to recent advancements.
- Details various LC separation modes (reversed-phase, ion-exchange, size-exclusion) and electromigration modes (capillary electrophoresis, micellar electrokinetic chromatography, etc.).
- Provides a comparative analysis of hyphenation technologies, including their pros and cons.
Conclusions:
- Multidimensional LC-E and E-LC separations significantly improve molecular resolution in complex matrices.
- The review offers a valuable resource for understanding and applying these advanced separation techniques.
Related Concept Videos
01:30Capillary Electrophoresis: Applications
1.6K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.6K
01:20Electrophoresis: Overview
4.6K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
There...
4.6K
01:05High-Performance Liquid Chromatography: Elution Process
1.8K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
1.8K
01:09Ion-Exchange Chromatography
2.6K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
2.6K
01:11High-Performance Liquid Chromatography: Introduction
3.9K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
3.9K
01:22Two-dimensional Gel Electrophoresis
8.0K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
8.0K