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Characterization of Escherichia coli causing community acquired urinary tract infections in Mexico City

Laura Belmont-Monroy1, Rosa María Ribas-Aparicio2, Armando Navarro-Ocaña3

  • 1Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional (IPN), Mexico City, Mexico; Laboratorio de Patogenicidad Bacteriana, Unidad de Hemato-Oncología e Investigación, Hospital Infantil de México "Federico Gómez", Mexico City, Mexico.

Diagnostic Microbiology and Infectious Disease
|December 6, 2016
PubMed

Insights

The O25-ST131 clone of uropathogenic Escherichia coli (UPEC) is prevalent in community-acquired urinary tract infections (UTIs) in Mexico City. Many strains carry extended-spectrum beta-lactamase genes, indicating antimicrobial resistance.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Uropathogenic Escherichia coli (UPEC) is a leading cause of urinary tract infections (UTIs).
  • The O25-ST131 clone is a globally significant UPEC strain, often associated with antibiotic resistance.
  • Understanding the prevalence and characteristics of UPEC clones is crucial for public health.

Purpose of the Study:

  • To identify the O25-ST131 clone within a collection of UPEC strains.
  • To determine the prevalence of extended-spectrum beta-lactamase (ESBL) genes in detected O25-ST131 isolates.
  • To investigate the association of the O25-ST131 clone with community-acquired UTIs in Mexico City.

Main Methods:

  • Analysis of 169 uropathogenic Escherichia coli strains.
  • Detection of the O25-ST131 clone using molecular methods.
  • Screening for extended-spectrum beta-lactamase genes.
  • Phylogroup determination.

Main Results:

  • The O25-ST131 clone was identified in 29 out of 169 UPEC strains (17.2%).
  • 44.8% of the O25-ST131 isolates harbored at least one extended-spectrum beta-lactamase gene.
  • Phylogroup D was the predominant phylogroup among the O25-ST131 strains.
  • The O25-ST131 clone showed an association with community-acquired UTIs in Mexico City.

Conclusions:

  • The O25-ST131 clone is present in UPEC strains causing community-acquired UTIs in Mexico City.
  • A significant proportion of these O25-ST131 strains carry ESBL genes, contributing to antimicrobial resistance concerns.
  • Further surveillance and characterization of UPEC clones are necessary for effective UTI management.
Keywords:
Escherichia coliMultiplex PCRST131 cloneSerogroupsUrinary infection

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