Lipoprotein profiling methodology based on determination of apolipoprotein concentration
Hiroaki Takeda1, Yoshihiro Izumi1, Atsumi Tomita2
1Division of Metabolomics, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812 8582, Japan.
Aim:
Abnormal lipid metabolism results in the alteration of lipid compositions in lipoproteins; therefore an accurate and quantitative analytical approach is required for the detailed structural characterization of lipoproteins. However, the specific lipid composition of each lipoprotein particle is poorly understood.
Materials & Methods:
Lipid composition of very-low-density lipoprotein and low-density lipoprotein particles derived from myocardial infarction-prone rabbits was determined by normalization of lipidomics data using apoB-100 levels.
Results:
The ratio of lipid levels between very-low-density lipoprotein and low-density lipoprotein particles was different according to not only lipid classes, but also phosphatidylethanolamine subclasses by applying our developed methodology to myocardial infarction-prone rabbits.
Conclusion:
Our novel analytical approach represents to be a potentially useful tool to obtain particle-specific lipid components of lipoproteins.
