Bradykinin inhibits a potassium M-like current in rat pheochromocytoma PC12 cells
A Villarroel1, N V Marrion, H Lopez
1Howard Hughes Medical Institute, Department of Neurobiology and Behavior, SUNY, Stony Brook 11794-5230.
Abstract:
We studied the action of bradykinin (BK) on ionic currents in fused pheochromocytoma PC12 cells under voltage-clamp in whole-cell mode, and on intracellular calcium using fura-2 BK induced the development of an outward current associated with an increase in intracellular calcium, followed by inhibition of an M-like current. The outward current was blocked by (+)-tubocurarine, and prevented when the calcium BAPTA or high concentrations of inositol 1,4,5-triphosphate were introduced into the cell, whereas the M-like current and its inhibition by BK remained unaffected. The protein kinase activator phorbol 12,13 dibutyrate partially reduced the M-current. M-current density did not substantially change after prolonged treatment with nerve growth factor.
More Related Videos
08:11Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique
Published on: November 11, 2022
07:19Recording of Inward Rectifying K+ Currents in Freshly Isolated Basilar Artery Smooth Muscle Cells by Patch Clamp Technique
Published on: February 7, 2025
Related Concept Videos
G-Protein Gated Ion Channels
Sensory organs,...
Drugs Acting on Autonomic Ganglia: Blockers
Antihypertensive Drugs: Action of β1 Blockers
Antihypertensive Drugs: Action of Calcium Channel Blockers
Antihypertensive Drugs: Angiotensin II Receptor Blockers
Antiarrhythmic Drugs: Class III Agents as Potassium Channel Blockers
