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Updated: Mar 10, 2026
Minimum Volume Vitrification of Immature Feline Oocytes
Published on: June 24, 2020
EFFECT OF NAC ON MOUSE GV OOCYTE SURVIVAL AND SUBSEQUENT EMBRYONIC DEVELOPMENT FOLLOWING VITRFICATION
S L Yue1, Y T Zhang, S W Wang1
1College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang, China.
Background:
Oocytes that survive cryopreservation may accumulate ROS which are known to bring harmful effects on embryonic development. NAC is an antioxidant which can be a supplement to reduce oxidative stress. However, whether NAC can improve the developmental competence of vitrified GV-oocytes remains unclear.
Objective:
The study was to investigate the effect of NAC on subsequent embryonic developmental competence of mice vitrified GV-oocytes.
Materials And Methods:
This study compared the effects of different concentration of NAC on the cleavage and blastocyst rates of mice vitrified GV-oocytes. Then the effects of NAC on mitochondria distribution, ROS level and embryonic development of vitrified oocytes were tested.
Results:
ROS activity of vitrified oocytes was significantly annihilated and mitochondrial distribution pattern was improved by 1.5 mM NAC (P<0.05). NAC supplementation throughout vitrification/warming and IVM media significantly improved the developmental competence of vitrified oocytes.
Conclusion:
Supplementation of NAC could partially overcome the damages by vitrification and improve the development ability of mice vitrified GV-oocytes.
