Metal-Organic Framework as a Protective Coating for Biodiagnostic Chips
Congzhou Wang1, Sirimuvva Tadepalli1, Jingyi Luan1
1Department of Mechanical Engineering and Materials Science, Institute of Materials Science and Engineering, Washington University in St. Louis, Saint Louis, MO, 63130, USA.
Advanced Materials (Deerfield Beach, Fla.)
|December 8, 2016
Summary
Zeolitic imidazolate framework-8 (ZIF-8) protects antibodies on nanostructures, maintaining their function during storage. A simple rinse restores biochip functionality for point-of-care applications.
Area of Science:
- Materials Science
- Biotechnology
- Nanotechnology
Background:
- Antibody-based biosensors are crucial for diagnostics.
- Antibody stability is a major challenge, especially in resource-limited settings.
- Plasmonic nanostructures enhance biosensor sensitivity.
Purpose of the Study:
- To develop a protective coating for antibodies on nanostructures.
- To enhance the stability and reusability of antibody-based biochips.
- To enable robust antibody storage for point-of-care diagnostics.
Main Methods:
- Growing zeolitic imidazolate framework-8 (ZIF-8) around antibodies.
- Anchoring antibody-nanostructure conjugates to a substrate.
- Testing antibody biorecognition after storage at various temperatures.
- Evaluating biochip functionality restoration via water rinsing.
Main Results:
- ZIF-8 coating preserved antibody biorecognition for several days at room and elevated temperatures.
- Stored biochips showed restored functionality after a simple water-rinsing step.
- The ZIF-8 protection strategy is compatible with antibody-functionalized plasmonic nanostructures.
Conclusions:
- ZIF-8 provides an effective protective layer for antibodies on nanostructures.
- This method significantly enhances antibody stability for diagnostic applications.
- The developed ZIF-8-protected biochip offers a promising solution for point-of-care diagnostics.
