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Graphene-Oxide-Based Enzyme Nanoarchitectonics for Substrate Channeling.
Motilal Mathesh1, Jingquan Liu2, Colin J Barrow1
1Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, 3217, Australia.
Chemistry (Weinheim an Der Bergstrasse, Germany)
|December 8, 2016
Summary
Enzyme immobilization on graphene oxide 2D scaffolds enhances cascade reaction efficiency through substrate channeling. Random enzyme arrangement on these hydrophobic surfaces achieves near-instantaneous reactions and stable glucose detection.
Area of Science:
- Biochemistry
- Materials Science
- Chemical Engineering
Background:
- Multi-enzyme cascade reactions benefit from controlled spatial organization for efficient substrate channeling.
- Scaffold-based enzyme immobilization is a key strategy to enhance reaction rates and overcome diffusion limitations.
Purpose of the Study:
- To investigate the impact of graphene oxide scaffolds on the activity of randomly immobilized multi-enzyme cascade reactions.
- To explore the role of scaffold hydrophobicity and enzyme architecture in achieving high product-conversion rates.
- To demonstrate the application of enzyme-scaffold systems in biosensing.
Main Methods:
- Co-immobilization of enzymes onto graphene oxide 2D scaffolds.
- Systematic study of hydrophobicity and enzyme architecture effects on cascade reaction performance.
- Fabrication of a bienzyme paper for glucose detection.
Main Results:
- Hydrophobicity of graphene oxide and enzyme architecture significantly influence product-conversion rates.
- Random enzyme immobilization on scaffolds enables direct molecular channeling, achieving transient times close to 0 seconds.
- The fabricated bienzyme paper exhibited high stability, reusability, and enhanced substrate channeling for glucose detection.
Conclusions:
- Random enzyme immobilization on 2D scaffolds is an effective strategy for efficient multi-enzyme cascade reactions.
- Graphene oxide's properties can be tuned to optimize enzyme activity and substrate channeling.
- Enzyme-scaffold systems offer promising applications in stable and reusable biosensors.
