Protective activity of Hertia cheirifolia extracts against DNA damage, lipid peroxidation and protein oxidation

Hertia cheirifolia L. (Asteraceae), a perennial shrub widely distributed in Northern Africa, is traditionally used to treat inflammatory disorders.

The protective effect of methanol (Met E) and aqueous (Aq E) extracts of Hertia cheirifolia against DNA, lipid and protein oxidation was investigated.

Different concentrations (50-1000 μg/mL) of Hertia cheirifolia aerial part extracts were examined against DNA, lipid and protein oxidation induced by H₂O₂+ UV, FeSO₄, and Fe³⁺/H₂O₂-ascorbic acid, respectively. The DPPH^•, metal ion chelating, reducing power and β-carotene bleaching tests were conducted.

Both extracts were rich in polyphenols, flavonoids and tannins, and were able to scavenge DPPH^• with IC₅₀ values of 138 and 197 μg/mL, respectively. At 300 μg/mL, Aq E exerted stronger chelating effect (99%) than Met E (69%). However, Met E reducing power (IC₅₀=61 μg/mL) was more than that of Aq E (IC₅₀=193 μg/mL). Both extracts protected from β-carotene bleaching by 74% and 94%, respectively, and inhibited linoleic acid peroxidation. The inhibitory activity of Aq E extract (64%) was twice more than that of Met E (32%). Interestingly, both extracts protected DNA against the cleavage by about 96-98%. At 1 mg/mL, Met E and Aq E restored protein band intensity by 94-99%.

Hertia cheirifolia exhibits potent antioxidant activity and protects biomolecules against oxidative damage; hence, it may serve as potential source of natural antioxidant for pharmaceutical applications and food preservation. This is the first report on the protective activity of this plant against biomolecule oxidation.