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Real time optical Biopsy: Time-resolved Fluorescence Spectroscopy instrumentation and validation
David S Kittle1, Fartash Vasefi1, Chirag G Patil1
1Cedars Sinai Medical Center, Department of Neurosurgery, Los Angeles, 90048, USA.
Scientific Reports
|December 9, 2016
Summary
Time-resolved fluorescence spectroscopy (TR-FS) can distinguish tumor from normal tissue during surgery. This novel TR-FS device accurately quantifies fluorophore concentrations in real-time, aiding surgical decisions.
Area of Science:
- Biomedical Optics
- Spectroscopy
- Surgical Technology
Background:
- Accurate tissue differentiation during surgery is crucial for complete tumor removal.
- Time-resolved fluorescence spectroscopy (TR-FS) shows promise for real-time tissue analysis.
Purpose of the Study:
- To design and validate a novel TR-FS device for differentiating tissue types.
- To assess the device's accuracy in quantifying fluorophore concentrations in mixtures.
Main Methods:
- Development of a novel TR-FS instrument for near real-time fluorescence lifetime acquisition.
- Validation using organic fluorescence dyes and mixtures to determine accuracy.
- Analysis of fluorescence lifetime decays for concentration unmixing.
Main Results:
- The TR-FS device achieved sub-20 picosecond accuracy in fluorescence lifetime recovery.
- Validated lifetime values closely matched published data for standard dyes.
- Demonstrated ability to quantify relative fluorophore concentrations in complex mixtures in near real-time.
Conclusions:
- The developed TR-FS system can accurately differentiate fluorophore concentrations in mixtures.
- This technology has potential for real-time tissue analysis during surgical procedures.
- Relative concentration information is encoded in multi-band fluorescence lifetimes.
