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Related Concept Videos

Transcription01:10

Transcription

158.1K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
158.1K
Riboswitches01:56

Riboswitches

10.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
10.0K

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Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry
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Thorium impact on tobacco root transcriptome.

Kateřina Mazari1, Přemysl Landa2, Sylva Přerostová3

  • 1Laboratory of Plant Biotechnologies, Institute of Experimental Botany AS CR, v.v.i., Rozvojová 263, 165 02 Prague 6, Lysolaje, Czechia; Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Kamýcká 129, Praha 6, Suchdol, 165 21, Czechia.

Journal of Hazardous Materials
|December 9, 2016
PubMed
Summary

Thorium exposure alters gene expression in tobacco roots, impacting stress responses and nutrient signaling. This research is key to understanding phytoremediation potential for thorium-contaminated sites.

Keywords:
Gene expressionMicroarrayNicotiana tabacumThoriumToxicity

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Area of Science:

  • Environmental Science
  • Plant Biology
  • Toxicology

Background:

  • Thorium contamination poses environmental risks due to radioactivity and toxicity.
  • Phytoremediation is a potential strategy for cleaning thorium-polluted areas.
  • Understanding plant responses to thorium is crucial for effective phytoremediation.

Purpose of the Study:

  • To investigate the transcriptomic response of tobacco roots to thorium exposure.
  • To identify genes and pathways affected by thorium in plants.
  • To provide insights into the mechanisms of thorium phytotoxicity and transport.

Main Methods:

  • Tobacco plants were exposed to 200μM thorium for one week.
  • Gene expression analysis was performed on root tissues.
  • Differential gene expression was identified using transcriptomic profiling.

Main Results:

  • Thorium exposure significantly altered gene expression, with 152 genes up-regulated and 100 down-regulated.
  • Affected pathways include jasmonic acid and salicylic acid signaling, abiotic/biotic stress responses, and phosphate starvation.
  • Genes potentially involved in thorium transport (e.g., ZIF2, PCR2, ABCG40) were identified.
  • Disruption of water homeostasis and iron metabolism was observed.

Conclusions:

  • Thorium significantly impacts plant molecular processes, including stress signaling and nutrient uptake.
  • The study identifies potential thorium transporter genes, aiding in phytoremediation research.
  • This work provides foundational knowledge on plant responses to thorium contamination.