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Performance Validated 8-Week Sanitation Interval for Mouse (Mus musculus) Wire-Bar Lids: ATP and CFU Outcomes.

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Developing a Performance Standard for Adequate Sanitization of Wire-Bar Lids.

Linna E Srn1, Louise K Hammarberg1, Robin J Kastenmayer1

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Rodent cage wire-bar lids can be used for 3 weeks without significant bacterial buildup, regardless of diet. This finding supports extending cleaning intervals for laboratory animal housing.

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Area of Science:

  • Laboratory animal science
  • Microbiology
  • Animal welfare

Background:

  • Wire-bar lids are critical components of rodent cage microenvironments, influencing animal health.
  • Current guidelines recommend bi-weekly lid changes but lack specific sanitization performance standards.
  • Assessing microbial burden on lids is essential for establishing evidence-based cleaning protocols.

Purpose of the Study:

  • To evaluate the bacterial and cellular burden on rodent cage wire-bar lids over a four-week period.
  • To determine if extended use impacts lid hygiene and potentially animal well-being.
  • To inform the development of a sanitization performance standard for wire-bar lids.

Main Methods:

  • Wire-bar lids were assessed for adenosine triphosphate (ATP) and bacterial load over four weeks.
  • Experiments included conventional cages with standard or high-fat diets and individually ventilated cages (IVC) with irradiated diets.
  • Lid usage duration was varied to simulate different cleaning schedules.

Main Results:

  • No significant differences in ATP or bacterial burden were observed on wire-bar lids used continuously for up to three weeks.
  • Diet type (standard pelleted, high-fat, or irradiated) did not significantly affect microbial load on the lids.
  • The findings suggest current usage patterns may not lead to a critical increase in microbial contamination within three weeks.

Conclusions:

  • Rodent cage wire-bar lids can be safely used for at least three weeks without a significant increase in bacterial or cellular burden.
  • This study provides data to support a potential revision of cleaning frequency recommendations for laboratory rodent housing.
  • Further research may explore longer-term usage and the impact of different sterilization methods.