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Related Experiment Video

Updated: Jun 21, 2026

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
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A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Published on: July 25, 2017

Performance Validated 8-Week Sanitation Interval for Mouse (Mus musculus) Wire-Bar Lids: ATP and CFU Outcomes.

Angelica J Petersson, Linnéa E Särén, Linda M Kroon

    Journal of the American Association for Laboratory Animal Science : JAALAS
    |June 19, 2026
    PubMed
    Summary

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    Developing a Performance Standard for Adequate Sanitization of Wire-Bar Lids.

    Journal of the American Association for Laboratory Animal Science : JAALAS·2016
    Same author

    The Polyunsaturated Fatty Acids Arachidonic Acid and Docosahexaenoic Acid Induce Mouse Dendritic Cells Maturation but Reduce T-Cell Responses In Vitro.

    PloS one·2015
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    Extending mouse wire-bar lid sanitation to 8 weeks is feasible without increasing organic or microbial load. This change can significantly reduce energy, water, and labor costs in laboratory animal facilities.

    Area of Science:

    • Laboratory animal science
    • Animal welfare and husbandry
    • Biosecurity and sanitation protocols

    Background:

    • Frequent sanitation of rodent cage accessories is resource-intensive.
    • Current guidelines recommend 2-week intervals for cage tops, but performance-based intervals are permissible with supporting data.

    Purpose of the Study:

    • To evaluate extending mouse wire-bar lid sanitation intervals to 8 weeks.
    • To assess the impact on organic residue and microbial load.
    • To determine potential resource savings.

    Main Methods:

    • A 10-week study involving 5 cage configurations.
    • Sampling of wire-bar lids at 2-week intervals (0-10 weeks).
    • Quantification of organic residue via ATP luminometry and microbial load via total plate count (TPC).
    Keywords:
    IVC, individually ventilated cagingRLU, relative light unitRODAC, replicate organism detection and countingTOST, two one-sided testsTPC, total plate count

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    Main Results:

    • All tested organic residue (ATP) and microbial load (TPC) values remained below prespecified action thresholds.
    • ATP levels were consistently <5% and TPC maxima <40% of action levels.
    • Biologic equivalence was demonstrated between 8-week and 2-week intervals, with confidence intervals within margins.

    Conclusions:

    • Extending wire-bar lid sanitation to 8 weeks is feasible without compromising cleanliness.
    • This extension is projected to yield substantial savings in energy, water, and labor.
    • Findings support site-specific, performance-validated sanitation intervals for laboratory animal housing.