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CD16 surface molecules regulate the cytolytic function of CD3CD16+ human natural killer cells
A Moretta1, G Tambussi, E Ciccone
1Istituto di Istologia ed Embriologia Generale, Università di Genova, Italy.
International Journal of Cancer
|October 15, 1989
Summary
Monoclonal antibodies targeting CD16 molecules can induce cell lysis. However, the anti-CD16 IgM antibody OKNK inhibits this lysis, suggesting CD16 receptors regulate immune cell responses.
Area of Science:
- Immunology
- Cell Biology
Background:
- Monoclonal antibodies (MAbs) targeting CD16 molecules can induce target cell lysis.
- Anti-CD2 MAbs also induce T lymphocyte activation and cytolytic function in specific lymphocyte subsets.
Purpose of the Study:
- To investigate the regulatory mechanisms in the activation and cytolytic function of CD3- CD16+ effector cells.
- To understand the role of CD16 receptor molecules in immune cell regulation.
Main Methods:
- Utilized anti-CD16 IgM antibody (OKNK MAb) to study regulatory mechanisms.
- Pre-treated effector cells with OKNK MAb to assess inhibition of target cell lysis.
- Compared effects of OKNK MAb on CD3- CD16+ and CD3+ CD16+ lymphocytes.
Main Results:
- OKNK MAb inhibited target cell lysis induced by anti-CD16 (IgG) MAbs and anti-CD2 MAbs.
- OKNK MAb also inhibited PHA-induced and spontaneous lysis in CD3- CD16+ lymphocytes.
- In CD3+ CD16+ clones, OKNK MAb selectively inhibited anti-CD16 MAb-induced triggering.
Conclusions:
- CD16 receptor molecules on CD3- CD16+ lymphocytes down-regulate responses to anti-CD2 MAbs and PHA.
- CD16 plays a regulatory role in the cytolytic function of these effector cells.