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Updated: Mar 10, 2026

Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue
Published on: May 25, 2011
Generation of human embryonic stem cells from abnormal blastocyst diagnosed with adrenoleukodystrophy
Qi Ouyang1, Xiaoying Zhou2, Jing Chen3
1Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China.
Abstract:
Human embryonic stem cell (hESC) line chHES-480 was derived from abnormal blastocyst diagnosed with adrenoleukodystrophy (ALD) after preimplantation genetic diagnosis (PGD) treatment. DNA sequencing analysis confirmed that chHES-480 cell line carried a hemizygous missense mutation c.1825G>A(p.Glu609Lys) of ABCD1 gene. Characteristic tests proved that the chHES-480 cell line presented typical markers of pluripotency and had the capability to form the three germ layers both in vitro and in vivo.
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